Development of a comprehensive diagnostic system for apple diseases using a DNA array
Author: Peter Sholberg, Karen Bedford, Dan O’Gorman, André Lévesque
Published: 2004
Summary: The objectives of this project were: 1. The objective of the project was to develop a DNA array diagnostic system for apple diseases that includes the major pathogens of apple found in the Pacific Northwest region of North America. The diagnostic system is based on a DNA array of species-specific DNA probes using reverse dot blot hybridization and allows rapid screening and identification of several fungal and bacterial pathogens in one test from various types of sample tissue. 2. In 2003-2004 the objectives were to refine the array to provide quantitative relative scale assessment of important apple pathogens useful for predictive monitoring management and storage decisions