Diagnostic system for apple diseases using a DNA array (Comparison of Real-time PCR and DNA array technologies).
Author: Peter Sholberg, Dan O’Gorman, Karen Bedford, André Lévesque
Published: 2004
Summary: The objectives of this report were: 1. Compare the sensitivity, specificity and reproducibility of DNA array, and Real-time PCR detection techniques for Powdery mildew & Fire blight. 2. Evaluate the usefulness of the techniques for monitoring and predictive purposes using fire blight and Powdery mildew predictive models as a reference in comparison to traditional monitoring. 3. Evaluate relative cost effectiveness of the various techniques for commercial development including potential applications to micro-array and microchip technologies.