Evaluation of apple lines overexpressing the Antioxidant APX
Author: Michael Wisniewski, Les Fuchigami
Published: 2005
Summary: The objectives of this project were: ???? Evaluation of three transgenic apple lines overexpressing APX. Evaluations will be conducted in both West Virginia (Kearneysville) and Oregon (Corvallis). Evaluation of material will be as self-rooted plants and as scions grafted on a commercial rootstock. ???? Evaluation will consist of monitoring growth parameters, photosynthetic and antioxidant parameters on lines grafted on commercial rootstocks, especially under conditions of environmental stress. ???? Continued characterization of the resistance of potted plants to environmental stresses such as drought, and high and low temperatures, and high light. ???? Construction of transgenic lines of apple overexpressing other antioxidant enzyme genes such as SOD (superoxide dismutase). ???? Characterization of seasonal expression of the APX gene, and enzyme activity in different tissues (leaves, stem, and bud). ???? Determining how overexpression of APX affects other parameters of the antioxidant system (SOD, glutathione reductase, catalase, etc.).
