Regulation of Farnesene Synthesis to Control Scald
Author: Bruce D. Whitaker, Steven W. Pechous, Nigel E. Gapper
Published: 2004
Summary: The objectives of this project were: (1) Obtain a genomic clone of the apple α-FS gene including the promoter and ethylene response element(s); (2) Isolate and characterize the promoter region from the genomic clone of the apple peel HMG2 gene and determine whether expression of HMG2 is regulated by ethylene; (3) Evaluate the correlation of ethylene-induced transcription of genes encoding enzymes in the α-farnesene synthetic pathway with accumulation of α-farnesene during storage, and with the incidence and severity of scald development; (4) Transform apple shoots with antisense or RNAi constructs of the α-FS gene (and the HMG2 gene if time allows) driven by either the CaMV 35S or apple α-FS promoter and test transgenic apple plants for suppression of α-farnesene production.
